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 | BioBall use in water and waste water testing
BioBall is an ideal QC reference sample for water and waste water testing.
Testing for bacteria in water is typically performed by –
- Passing a water sample through a membrane filter
- Transferring the filter to a media
- Incubating the media
- Identifying any colonies that grow
Regulations on the quality control of water and waste water testing vary from country to country but usually the procedure is performed periodically with a quality control sample containing less than 100 cfu.
BioBall can be added directly to a sterile water sample which is run through the standard test method. As the number of bacteria in BioBall is known, the test recovery can easily be determined.
BioBall is US EPA approved as a control in the following US EPA methods:
- Method 1600: Enterococci in Water by Membrane Filtration Using membrane-Enterococcus Indoxyl- -D-Glucoside Agar (mEI)
- Method 1603: Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar (Modified mTEC)
- Method 1682: Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium
- Method 1103.1: Escherichia coli (E. coli) in Water by Membrane Filtration Using membrane-Thermotolerant Escherichia coli Agar (mTEC)
- Method 1106.1: Enterococci in Water by Membrane Filtration Using membrane-Enterococcus-Esculin Iron Agar (mE-EIA)
BioBall is also ideal for media QC. For solid media plates, BioBall can be tipped directly onto the media, re-hydrated with 100 μL of sterile water, spread and incubated. See application notes for media QC procedures for spread plates, pour plates and liquid media.
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